bio rad c1000 touch thermal cycler (Bio-Rad)

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Bio-Rad bio rad c1000 touch thermal cycler
Bio Rad C1000 Touch Thermal Cycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 494 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c1000+touch+thermal+cycler/C1000+Touch+Firmware+Update/pmc13049409-265-16-16
Average 99 stars, based on 494 article reviews

bio rad c1000 touch thermal cycler - by Bioz Stars, 2026-10

99/100 stars

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Incubation:

Article Title: Methods and compositions for treatment of pancreatic cancer
Article Snippet: .. After GEM generation, the samples were subjected to an incubation at 53° C. for 45 min in a C1000 Touch Thermal cycler with 96-Deep Well Reaction Module (Bio-Rad, Hercules) to generate poly A cDNA barcoded at the 5′ end by the addition of a template switch oligo (TSO) linked to a cell barcode and Unique Molecular Identifiers (UMIs). .. GEMs were broken, and the single-strand cDNA was cleaned up with DynaBeads MyOne Silane Beads (Thermo Fisher Scientific, Waltham, MA).

Real-time Polymerase Chain Reaction:

Article Title: Circulating microRNA Profiles as Diagnostic Tools for High-Grade Cervical Lesions and HPV Genotype Stratification.
Article Snippet: .. Before analysis in a C1000 Touch Thermal Cycler (Bio-Rad Laboratories Inc., Hercules, CA, USA, Cat. No. 1851196) with a CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories Inc., Cat. No. 1845097), the PCR plates were shortly centrifuged. ..

Polymerase Chain Reaction:

Article Title: Investigating the functional components of YAP condensates
Article Snippet: Yes-associated protein (YAP) condensates are critical for cell survival under hyperosmotic stress, yet how these condensates execute their specific functions remains incompletely understood.. Here, we employed proximity-based proteomics to identify YAP-interacting proteins in both the diffuse and condensate-forming states.. Upon YAP condensate formation, the composition of YAPinteracting proteins changed markedly.

Article Title: Intrinsic regulation of intestinal stem cell fate and homeostasis by Tet
Article Snippet: Individual nuclei were collected into one 1.5ml RNase-free Eppendorf tube with 200μl 1x PBS with 0.5% BSA (RNase inhibitor added) as the receiving buffer. .. All PCR reactions were performed using the BioRad C1000 Touch Thermal cycler with a 96-deep Well Reaction Module. ..

Cloning:

Reverse Transcription:

Article Title: Longitudinal localization of leukaemic stem cells between the metaphysis and central marrow governs their behaviour
Article Snippet: .. Reverse transcription and library preparation were performed on C1000 Touch Thermal cycler with 96-Deep Well Reaction Module (Bio-Rad). .. Amplified cDNA and final libraries were evaluated on an Agilent Bioanalyzer using a High Sensitivity DNA Kit (Agilent Technologies).

Article Title: Longitudinal localization of leukaemic stem cells between the metaphysis and central marrow governs their behaviour.
Article Snippet: .. Reverse transcription and library preparation were performed on C1000 Touch Thermal cycler with 96-Deep Well Reaction Module (Bio-Rad). .. Amplified cDNA and final libraries were evaluated on an Agilent Bioanalyzer using a High Sensitivity DNA Kit (Agilent Technologies).

Single Cell:

Article Title: Single-cell transcriptomic and chromatin accessibility atlas of peripheral blood mononuclear cells reveals immune cell heterogeneity and breed-specific characteristics in Duroc and Meishan pigs.
Article Snippet: Vortexed Chromium Single Cell ATAC Gel Beads (10x Genomics; 2000132) and Partitioning Oil (10x Genomics; 220088) were also loaded onto the same Chromium Chip E and placing into a Chromium Single Cell Controller instrument (10x Genomics). .. Resulting single-cell GEMs were collected at the completion of the run (~7 min) and linear amplification was performed in a C1000 Touch Thermal cycler with 96-Deep Well Reaction Module (Bio-Rad; 1851197). .. Emulsions were coalesced using the Recovery Agent (10x Genomics; 220016), then subjected to Dynabeads (10x Genomics; PN-2000048) and SPRIselect reagent (Beckman Coulter; B23318) bead clean-ups.

Amplification:

Purification:

Article Title: Single-cell transcriptomics of bronchoalveolar lavage reveals divergent macrophage subpopulations and trajectories in interstitial lung disease
Article Snippet: Single-cell suspensions for samples pertaining to BAL_scRNA_sample# were loaded onto 10X Genomics Single Cell 3′ Chips along with the RT mastermix as per the manufacturer’s protocol for the Chromium Single Cell 3′ Library (v2, PN-120233; 10X Genomics), to generate single-cell gel beads in emulsion. .. RT was performed using a C1000 Touch Thermal Cycler with a Deep Well Reaction Module (Bio-Rad) as follows: 55°C for 2 h; 85°C for 5 min; hold 4°C. cDNA was recovered and purified with DynaBeads MyOne Silane Beads (catalog no. 37002D; Thermo Fisher Scientific) and SPRIselect beads (catalog no. B23318 ; Beckman Coulter). ..

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